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Fig. 2 | Virology Journal

Fig. 2

From: Genetic determinants of Nef-mediated CD4 and HLA class I down-regulation differences between HIV-1 subtypes B and C

Fig. 2

Representative flow cytometry plots of assay controls and Nef expression. Plots show cell-surface expression of CD4 or HLA-I (y axis) in cells transfected with empty plasmid (∆ Nef, negative control) and plasmid expressing SF2 Nef (positive control). Green fluorescent protein (GFP) expression is shown on the x axis and is an indicator of cells successfully transfected with plasmids. CD4 expression was measured in the lower third of GFP-expressing cells and HLA-I expression was measured in all GFP-expressing cells (gates shown on plots)

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