Fig. 1

Representative RFLP patterns of HCMV gB, gN and gO glycoproteins in the studied population. RFLP analysis was performed on the PCR-amplified gB (a), gN (b) and gO (c) sequences obtained from the patient-derived HCMV strains. Each sample was digested with restriction enzyme combinations as detailed in Tables 1, 2 and 3. In the upper side of panels a (gB) and b (gN), the numbers “1, 2” (panel a), and “1, 2, 3” (panel b) indicate the restriction enzymes used (gB: 1 = RsaI; 2 = HinfI; gN: 1 = SacI; 2 = ScaI; 3 = SalI). A single digestion with HpaII was used to characterise the gO gene products (panel c). The genotypes corresponding to each digest are displayed at the top of the lanes. Lanes MW molecular weight markers