Fig. 1

SAMHD1 overexpression promotes PMA-induced differentiation-related morphological phenotype of THP-1 cells. THP-1 control cells and monoclonal cell lines stably expressing HA-tagged SAMHD1 (wild-type or the HD/AA catalytically inactive mutant) or shRNA targeting SAMHD1 were differentiated by addition of 65 nM of PMA in the culture medium (2.10^5 cells/ml). 24 h after PMA removal, cells were left in PMA-free medium for several days. (a) Cell morphology was observed with a Zeiss 5 microscope (Gx20). The pictures shown here were taken 72 h after PMA addition. The results are representative of three independent experiments. (b) For each cell line, cytoplasmic extensions of cells 72 h after PMA addition were counted and normalized with regards to cell numbers (approximately 300 cells in each condition). The cytoplasmic extensions ratios obtained result from two independent experiments