Fig. 2
From: Duck enteritis virus UL54 is an IE protein primarily located in the nucleus
Preparation of anti-UL54 polyclonal antibody and UL54 expression analysis in DEV-infected cells. a UL54 protein was purified through gel and electric elution. b The anti-UL54 protein serum was cursorily purified using saturated ammonium sulfate. c Western blot analysis of the UL54 protein using rabbit anti-DEV-positive serum (lane 1) and rabbit negative control serum (lane 2). d Western blotting was used to analyze the lysates of DEV-infected DEFs harvested at different time points