Fig. 2

Microscopic analysis of the effect of PEG 6000 on HSV-1 infection of receptor-negative cells. B78 cells (a-d) or CHO cells (e-h) were infected with HSV-1 strain KOS (MOI of 10) (b, d, f, h) or remained uninfected (a, c, e, g) for 2 h at 4 °C. Cultures were treated with PEG 6000 (c, d, g, h) or mock-treated (a, b, e, f). At 24 h post-infection (p.i.), cultures were fixed with ice-cold methanol-acetone solution (2:1 ratio) for 20 min at −20 °C and air dried. HSV antigen-positive cells were detected by immunoperoxidase staining with anti-HSV polyclonal antibody HR50 (Fitzgerald Industries, Concord, MA) [75]. Magnification, 5X