Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: Polyethylene glycol-mediated fusion of herpes simplex type 1 virions with the plasma membrane of cells that support endocytic entry

Fig. 1

PEG-mediated entry of HSV-1 into cells that are resistant to entry and that normally mediate entry via an endocytosis pathway. HSV-1 strain KOS was bound to B78 cells (a) or CHO cells (c) (MOI of 10) for 2 h at 4 °C. Cell bound virus was treated with PEG 6000 (+ PEG) or mock-treated (− PEG). At 7 h post-infection, beta-galactosidase activity was detected as an indication of viral entry [74]. b, d HSV-1 KOS was bound to B78 and B78-nectin-1 cells (b) or CHO and CHO-nectin-1 cells (d) for 2 h at 4 °C at the indicated MOIs ranging from 0.5 to 10. B78 and CHO cell samples were treated with PEG and B78-nectin-1 and CHO-nectin-1 samples were mock treated. Beta-galactosidase activity was measured at 7 h p.i. b, d To allow a more direct comparison between samples of disparate reactivity, a fraction of the B78-nectin-1 and CHO-nectin-1 samples was measured. Values shown represent the beta-galactosidase activity from equivalent cell numbers. Each value is the mean of quadruplicate determinations with standard deviation. One representative experiment of at least three independent experiments is shown

Back to article page