Fig. 3

Phylogenetic analyses of global PEDV strains based on nucleotide sequences of the spike genes (a) and full-length genomes (b). A putative similar region of the spike protein and the complete genome sequence of TGEV was included as an outgroup in each panel. Multiple sequence alignments were performed using ClustalX 2.0 program and the phylogenetic tree was constructed from aligned nucleotide sequences using the distance-based neighbor-joining method of MEGA5.2 software. Numbers at each branch represent bootstrap values greater than 50 % of 1000 replicates. Names of the strains, countries and years of isolation, GenBank accession numbers, genogroups, and subgroups are shown. PEDV isolates identified in different countries are indicated by corresponding symbols: Europe (solid triangles), South Korea (sold circles), Thailand and Vietnam, (sold diamonds), and the United States (solid squares). Scale bars indicate nucleotide substitutions per site