Fig. 4

IL-27 ameliorates CVB3-induced myocarditis by inhibiting Th17 cells. a Representative FACS profile of Th17 cells in the spleen of CVB3 infection mice treated with rmIL-27 or Anti-IL-27 on day 7 post infection. Cells were gated on CD3⁺CD4⁺ T cells. b Statistical analysis of the percentage of Th17 cells. c Expression of Th17 cells characteristic cytokines, IL-17A, IL-22, and key transcription factor ROR-γt in cardiac tissues on day 7 after CVB3 infection were detected by qRT-PCR. d The serum levels of proinflammatory cytokines IL-17A, IFN-γ, IL-6 and TNF-α in CVB3 infection mice were determined by ELISA. Normal, mice not-infected with the virus. Data are showed as the means ± SEM. ^* P<0.05, ^** P<0.01, ^*** P<0.001