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Fig. 4 | Virology Journal

Fig. 4

From: The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells

Fig. 4

The role of NF-κB pathways on apoptosis of Huh-7-HBX cells induced by TRAIL. a Huh-7-HBX cells was transfected with shRNA plasmids for DR4 and DR5, and 48 h post-transfection, the expression of DR4 and DR5 proteins was measured by western blotting analysis. Huh-7-HBX cells transfected with control plasmid of shRNA were named as siCON cells, Huh-7-HBX cells transfected with shRNA plasmids for DR4 and DR5 (pGPU6/GFP/Neo-DR4 and pGPU6/GFP/Neo-DR5) were as siRNA cells. b After Huh-7-HBX cells transfected with shRNA plasmids for DR4 and DR5, and control plasmids for 48 h, the apoptosis was detected in Huh-7-Mock cells, Huh-7-HBX cells, and Huh-7-HBX cells transfected with different plasmids. c After the cells treated with 100 ng/mL TRAIL and 10 μM BAY11-7082 for 24 h, the apoptosis of cells was detected by flow cytometry analysis

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