Fig. 2
From: Central memory CD4+ T cells are preferential targets of double infection by HIV-1
a Cells infected with viruses expressing EGFP, mCherry, or both can be distinguished by flow cytometry. Representative data (1 of 9) of unstimulated primary CD4+ T cells infected with NL4-3-ΔE-EGFP and/or NL4-3-ΔE-mCherry reporter viruses pseudotyped with the primary X4-tropic isolate JOTO.TA1.2247. b Frequencies of EGFP+/mCherry-, EGFP-/mCherry+, and EGFP+/mCherry + cells 72 h after infection of unstimulated CD4+ T cells with EGFP and mCherry viruses, normalized by MOI. c For a given percentage of single positive cells (P(1), EGFP+mCherry- and EGFP-mCherry+), the expected frequency of double positive cells (P(2)) can be predicted by Poisson distribution. d Comparison of actual frequencies of double positive cells measured experimentally and frequences expected by Poisson distribution. The data are from experiments on 9 different healthy controls