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Table 2 Real-time PCR primers of virus genes (RSV and RBSDV) used in this study

From: Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR

Gene name

Primer sequence (5′ → 3′)

Amplicon length (bp)

Target genes

CP-F

TGCAGAAGGCAATCAATGACAT

150

RSV NCP

CP-R

TGTCACCACCTTTGTCCTCAA

 

SP-F

CCTGTTAGGAGGTGAAGATGATGA

180

RSV SP

SP-R

GCTCTCAGCCTTAGCCATCTTG

 

P5-1-F

GTTTACGGTGGTGCAATTTTCA

150

RBSDV P5-1

P5-1-R

AGGCTTTCCTTCACTAACTTCTGACT

 

P9-1-F

TGGTGCTTCTCGTCAAACTGTCT

100

RBSDV P9-1

P9-1-R

GCCAACAATTCGTGTCCTGAA

 
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Virology Journal

ISSN: 1743-422X