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Table 1 Primers used in this study

From: Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

Genes

Primer name

Sequencea

Precursor polyprotein (PP)

LCPP-F

5′-CGG AAT TCC Gat gGAC AAA CCT GCA AGA TC-3′

 

LCPP-R

5′-CCA AGC TTG GTC ACT CAA GGT CCT CAT-3′

VP4

LCVP4-F

5′-CGG CTA GCC Gat gAG GAT AGC TGT G-3′

 

LCVP4

5′-GGG GTA CCC CTC ATT TGA TAA ACG TCG C-3′

  1. aLower case letters indicate ATG codons. Underlined sequences indicate the restriction enzyme sites