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Fig. 2 | Virology Journal

Fig. 2

From: Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

Fig. 2

Biochemical analysis of proteins expressed by the recombinant baculovirus. a Western blot analysis of VLP antigens: lane 1, VLP antigen produced by rBac-PP; lane 2, VLP antigen produced by rBac-PP/VP4, b Antigenic reactivity of VLP antigens measured by DAS-ELISA. VLP antigens produced by rBac-PP and rBac-PP/VP4 were independently applied to DAS-ELISA to compare their R63 MAb reactivity with the R63 MAb reactivity of VP2 of IBDV. Serial dilution of VLP antigens were used to measure the titers of the expressed VP2 protein in VLPs

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