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Fig. 1 | Virology Journal

Fig. 1

From: Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

Fig. 1

Strategy for construction of recombinant baculovirus transfer vector. a A schematic representation of the IBDV segment A showing regions amplified (solid lines) and primer positions (arrows). Amplification of PP and VP4 genes of IBDV was performed by RT-PCR. b Construction of a recombinant single (pFastBac-PP) or dual (pFastBac-PP/VP4) expression vector containing the PP and VP4 genes of IBDV. c A schematic of the strategy used for the production of VLP antigen induced by recombinant baculovirus Bac-PP/VP4 in Sf9 cells

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Virology Journal

ISSN: 1743-422X