Fig. 5

Subcellular localization of the GFP-Bm64 fusion protein in BmN cells. a Schematic diagram of the construction of recombinant viruses. A gfp-bm64 chimera under the control of the polyhedrin promoter was inserted into the polh locus of the vBm^64KO bacmid to generate vBm^GFP-Bm64. The control virus vBm^GFP was constructed by transposing gfp under control of the polyhedrin promoter into the vBm^64KO bacmid. b Microscopy images of BmN cells infected with vBm^GFP-Bm64 and vBm^GFP. BmN cells were infected with vBm^GFP-Bm64 or vBm^GFP at an MOI of 1 and were observed for fluorescence by laser scanning microscopy at 36 or 72 h p.i