Fig. 4

IRF-3 is not activated in poliovirus-infected cells. a. ISG54 mRNA levels in infected cells. Total RNA extracted from uninfected, poliovirus-infected or poly(I:C) treated cells at the indicated times post-infection was analyzed by qRT-PCR with primers for both interferon stimulated gene 54 (ISG54) and β-actin mRNAs. The amount of ISG54 mRNA was normalized to the amount of β-actin mRNA. The fold induction of ISG54 mRNA relative to uninfected controls is shown. Error bars indicate the standard error of the results from triplicate wells. hpi: hours post infection. b. Homodimerization of IRF-3. Whole cell lysates prepared from cells that were mock-infected or had been infected with poliovirus for the indicated amount of time or treated with poly(I:C) for 3.5 h were analyzed by native PAGE followed by immunoblot to detect IRF-3. The monomeric and dimeric forms of IRF-3 are indicated