Fig. 4
From: Eastern equine encephalitis virus in mice II: pathogenesis is dependent on route of exposure
Immunohistochemical findings for cleaved-caspase 3 (a-d) and LC3BII (e-f) in mice after AE infection with EEEV strain FL93-939. Mice were infected with approximately 100LD50 and were euthanized at specified time points (n = 5). While cleaved-caspase 3 antigen was detected in very few cells in the olfactory bulb (a magnification X400), there was increased antigen detected in the olfactory bulb by 2 dpi (b magnification X100). The increase in cleaved-caspase 3 antigen detection in the olfactory bulb continued through the study and was present in clusters of cells at 4 dpi (c magnification X100). Cleaved-caspase 3 antigen was present within the cerebrum, primarily within the piriform cortex (d magnification X100) and specifically within cells with karyorrhexis (d inset, arrow) as well as within some inflammatory cells within the meninges. The LC3BII antigen was detected in low numbers of neurons within the olfactory bulb, frontal cortex (e magnification X100), and midbrain at 3–4 dpi. The antigen was cytoplasmic and punctate in affected cells (f magnification X400, arrow)