Interferon lambda inhibits dengue virus replication in epithelial cells

Table 1 Primers used in the present study

Gene name and accession number in GenBank	Sequence (5′–3′)	UPL sequence (UPL#)^a	Amplicon bp	Alignment^b
MX1	TCCAGCCACCATTCCAAG	TTCTCCTG (2)	60	868–885
NM_001144925.2	CAACAAGTTAAATGGTATCACAGAGC	TTCTCCTG (2)	60	927–902
OAS1	GGTGGAGTTCGATGTGCTG	CCAGGGCA (37)	64	538–556
XM_006719434.1	AGGTTTATAGCCGCCAGTCA	CCAGGGCA (37)	64	601–582
IFN-β	CTTTGCTATTTTCAGACAAGATTCA	CTGGCTGG (20)	69	336–360
NM_002176.2	GCCAGGAGGTTCTCAAGAAT	CTGGCTGG (20)	69	404–389
IFN-λ1	TCCTAGACCAGCCCCTTCA	^c	73	435–453
NM_172140.1	GTGGGCTGAGGCTGGATA	^c	73	507–490
IFN-λ2	CCTGGTGGACGTCTTGGA	^c	84	406–423
NM_172138.1	GTGGGCTGAGGCTGGATA	^c	84	489–472
IFN-λ3	AGGGCCAAAGATGCCTTAG	^c	123	167–185
NM_172139.2	CAGCTCAGCCTCCAAAGC	^c	123	298–281
HPRT1	–	–	127	–
NM_000194.2^d	–	–	127	–

^aUPL: Universal Probe Library, Roche Life Science. Number of the probe is in parentheses
^bPosition of primers in the corresponding gene sequences
^cSybrGreen dye was used
^dSpecific gene positions and sequences of Applied Biosystems primers and probes are not disclosed

ISSN: 1743-422X