Fig. 2

Permissivity of C33-A cells to DENV-2 infection. Cells were infected with dengue virus (MOI = 0.1) for 2 h, maintained for 24 h, washed, and fixed with methanol-acetone. The virus was detected using antibodies against the viral pr-M protein and a Texas Red secondary antibody and observed with fluorescence microscopy. a–c DENV-2-infected cells. d–f Mock-infected cells. a, d White light. b, e Fluorescence, (c, f) Merged images. Bars = 30 μm