Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4

Table 2 Flow cytometric analysis for proportion of T lymphocyte subpopulations in peripheral blood of pigs

Marker	14^a	28^a	3^b	7^b	14^b	21^b	28^b
CD3 ⁺
Group1	56.4 ± 4.5	61.1 ± 8.6	65.6 ± 3.6	66.4 ± 5.0	57.7 ± 4.7	58.7 ± 7.0	69.8 ± 7.9
Group2	51.9 ± 5.6	49.0 ± 6.7	53.9 ± 6.8	59.9 ± 3.9	51.8 ± 8.4	54.4 ± 5.8	58 ± 6.6
Group3	52.7 ± 3.9	49.8 ± 10.9	44.76 ± 6.3	59.6 ± 8.8	61.1 ± 5.2	57.2 ± 7.3	51.4 ± 8.3
Group4	50.8 ± 2.1	49.8 ± 6.4	48.3 ± 7.0	52.6 ± 8.7	51.8 ± 10.7	50.6 ± 9.7	52.4 ± 6.8
CD4 ⁺
Group1	18.6 ± 3.4	15 ± 4.1	9.7 ± 3.8	4.6 ± 0.3	4.3 ± 4.2	1.9 ± 0.6	4.1 ± 1.2
Group2	17 ± 1.2	16.5 ± 8.4	5.9 ± 2.9	5.2 ± 5.4	6.3 ± 2.3	1.33 ± 0.9	4.2 ± 2.5
Group3	28.7 ± 0.2	24.9 ± 8.2	11.6 ± 5.5	3.1 ± 1.5	8.0 ± 5.3	20 ± 6.3	22.7 ± 2.2
Group4	23.6 ± 4.3	30.7 ± 5.4	26.5 ± 3.7	32.4 ± 0.2	26.3 ± 3.5	19.6 ± 2.8	23.1 ± 3.3
CD8 ⁺
Group1	30.3 ± 5.3	33.3 ± 8.1	27.6 ± 6.5	64.8 ± 8.2	50.7 ± 6.6	53.0 ± 9.2	46 ± 0.2
Group2	27.5 ± 5.4	35.5 ± 7.3	26.4 ± 3.0	58.8 ± 9.1	48.6 ± 2.0	51.8 ± 11.2	50.1 ± 4.7
Group3	28.0 ± 4.5	29.1 ± 7.5	13.5 ± 2.1	24.5 ± 6.2	29.7 ± 9.5	38.8 ± 3.2	30 ± 3.6
Group4	29.7 ± 6.4	32.7 ± 3.0	29.3 ± 2.6	29.5 ± 8.7	30.2 ± 8.9	32.4 ± 3.3	36.7 ± 0.5
CD4 ⁺ CD8 ⁺
Group1	29.4 ± 7.8	23.9 ± 8.6	32.9 ± 3.1*	39.1 ± 2.6*	28.3 ± 7.1	25.4 ± 6.5	31.4 ± 7.7
Group2	30.5 ± 4.2	21.7 ± 9.7	18.3 ± 5.2	26.4 ± 4.5	17.2 ± 7.4	26.0 ± 7.9	29.5 ± 3.8
Group3	18.9 ± 0.1	18.5 ± 7.0	8.7 ± 3.0	7 ± 5.7	13.2 ± 1.2	19.2 ± 6.9	18.1 ± 5.3
Group4	20.5 ± 4.3	22.8 ± 3.1	28 ± 4.8	26.4 ± 10.2	25.7 ± 8.0	23.5 ± 6.5	28.9 ± 4.1

Results are expressed as mean (%) ± SD. Group 1: CH-1R/pIL-4 + Challenged; Group 2: CH-1R + Challenged; Group 3: Challenged; Group 4: Mock. *p < 0.05
^aDays post- immunization (DPI); ^bDays post-challenge (DPC)

ISSN: 1743-422X