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Table 1 Primers used for construction and identification of recombinant PRRSV infectious clones

From: Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4

Primers

Nucleotide sequence (5'-3')

Sense

F1-1

TTAACCAGAGTTTCAGCGGAACAATGGGTCTCACCTCCCAAC

+

F1-2

GCGATCGCTGATGGTTCCGTGGCAACCCCTTTAACCAGAGTTTCATG

+

R1

CGACGCGTCGTCAACACTTTGAGTATTTCTCCTT

-

F2

ATACTGTGCGCCTGATCCGC

+

R2

TCGCCAATTAAACTTTACCCCCACA

-

  1. The italics in the sequence denote the restriction enzyme sites (Asis I, Mlu I)
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Virology Journal

ISSN: 1743-422X