Fig. 3
From: Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4
Humoral and cell-mediated immunity in pigs infected with the recombinant and the parental virus. a PRRSV-specific serum antibodies were measured with the IDEXX HerdChek® PRRSV ELISA 2XR Kit. S/P ratios of greater than 0.4 are considered positive. S/P = (Sample A (650)-Negative Control) / (Positive Control- Negative Control). Parental and recombinant virus groups were compared to the challenged control group. **denotes p < 0.01; *** represents p < 0.001. b PBMCs were collected from the piglets stimulated with purified PRRS virions. PHA was the positive control. After 72 h of stimulation, the cells were treated with WST-8 for 2 h and the OD450 values were determined to calculate the stimulation index (SI). IFN-γ(c) and IL-4 (d) concentrations in the supernatants of PBMC cultures were tested after 72 h of stimulation using commercial ELISA Kits. *indicates p < 0.05. Data represent the mean ± SD of five pigs from each group