Fig. 2
From: Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4
Characterization of recombinant PRRSV expression of the pIL-4 gene in Marc-145 cells. a IFA detection of pIL-4 and N expression in Marc-145 cells infected with parental virus or recombinant virus. Original magnification 200×. b Western blot detection of pIL-4 and N protein in cell lysates of Marc-145 cells infected with recombinant virus and parental virus. c Growth curves of the recombinant and the parental virus in Marc-145 cells. d Kinetics of pIL-4 accumulation in the supernatants of Marc-145 cells infected with the recombinant virus vs. parental virus or mock control. Data are represented as the mean of three independent experiments