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Fig. 2 | Virology Journal

Fig. 2

From: Up-regulation of A20/ABIN1 contributes to inefficient M1 macrophage polarization during Hepatitis C virus infection

Fig. 2

HCV antigen pre-stimulation influences macrophage polarization in vitro. THP-1 cells were incubated with or without HCV antigens (HCVcc, core protein or NS3/4A) after PMA stimulated macrophage differentiation. Adherent cells were then treated with LPS/IFN-γ medium for M1-polarization before analysis. TNF-α (a), TfR1 (b), CD163 (c), CD23 (d), MMPs (e) and CD100 (f) expression levels were measured by RT-PCR. IL-10 and IL-12 expression levels from the culture medium were measured by ELISA (g)

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Virology Journal

ISSN: 1743-422X