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Fig. 5 | Virology Journal

Fig. 5

From: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

Fig. 5

Denaturing gel electrophoresis of total RNA isolated from different woody plants and strawberry using Spectrum™ Plant Total RNA kit (Sigma) (a) and Plant/fungi total RNA kit (Norgen BioTek) (b). 100 mg of young leaves from each plant as indicated were used in RNA isolation. Homogenization of leaf tissues was done using an extraction bag from BIOREBA. This experiment was conducted twice and consistent results were obtained. Only one set of samples is shown here. 7.5 μl of RNA was loaded in 1.2 % of agarose gel. The total RNA yield (μg), A260/A280 and A260/A230 ratios are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows

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Virology Journal

ISSN: 1743-422X