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Fig. 4 | Virology Journal

Fig. 4

From: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

Fig. 4

Effects of tissue homogenization and extraction methods on the RNA isolation from grapevine leaves. Young and mature leaves were homogenized by grinding in liquid nitrogen with mortar and pestle (Method A), or in BIOREBA extraction bag with lysis buffer (Method B), and then used to isolate total RNA with Plant/fungi total RNA kit (Norgen) and Spectrum™ Plant Total RNA kit (Sigma). Shown are the averages of total RNA yields of two replicates and their standard deviation. A260/A280 (on the left) and A260/A230 (on the right) ratios averaged from two replicates are given on the top of RNA yield bar

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Virology Journal

ISSN: 1743-422X