Fig. 3
RT-qPCR detection of GRSPaV from total RNA isolated from grapevine leaves. Total RNA was isolated from V. vinifera var. Chardonnay using Spectrum™ Plant Total RNA kit (Sigma), Plant/fungi total RNA kit (Norgen) and AccuPrep viral RNA extraction kit (Bioneer). cDNA prepared with oligo d(T) on 2 μg of total RNA were subjected to SYBR Green quantitative real-time PCR with primers targeting GRSPaV capsid protein gene, grape actin1 and the gene encoding ubiquitin-60S ribosomal protein L40-2 (Additional file 1: Table S1). Shown is the amplification plot (a) and melt curve (b) from cDNAs prepared from RNAs isolated with Sigma (blue), Norgen (purple) and Bioneer (green). The Cq and melting temperature (Tm) of two technical replicates for all the samples and genes are given in the table below