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Fig. 2 | Virology Journal

Fig. 2

From: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

Fig. 2

RT-PCR detection of GRSPaV from RNAs isolated with Spectrum™ Plant Total RNA kit (Sigma). a Agarose gel analysis of RT-PCR products with primers RSP21 and RSP22 targeting the capsid protein gene (Additional file 1: Table S1) on total RNA extracts from V. vinifera var. Chardonnay (lane 1), V. riparia from Quebec (lane 2), V. riparia from Manitoba (lane 3), V. vinifera var. Riesling (lane 4) and V. vinifera var. Thompson seedless (lane 5). Lane M: molecular size marker (bp); lane 6: water control. b Agarose gel analysis of RT-PCR products using total RNA extracts from V. vinifera var. Syrah. Lanes 1–6: leaf samples from six individual vines. Lane 7: water. Lane M: molecular size marker (bp)

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Virology Journal

ISSN: 1743-422X