Proteolytic cleavage analysis at the Murray Valley encephalitis virus NS1-2A junction

Table 2 Recovery of MVEV NS1-2A cleavage site mutants

Virus	Octapeptide motif and mutation ^a	Plaque size ^b (Fig. 3)	NS1-2A cleavage (from Table 1)
rMVEV	L-V-K-S-R-V-Q-A ↓	4 mm	88 %
rP3-Gly	L-V-K-S-R-G-Q-A ↓	4 mm	83 %
rP8-Ala	A-V-K-S-R-V-Q-A ↓	4 mm	80 %

^aMutations introduced into the octapeptide are in bold and underlined, and arrow denotes the NS1-2A cleavage site
^bPlaque size on Vero cell monolayers stained with neutral red at 4 days pi and observed 16 h later