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Fig. 4 | Virology Journal

Fig. 4

From: Proteolytic cleavage analysis at the Murray Valley encephalitis virus NS1-2A junction

Fig. 4

Growth and RNA synthesis analysis. a Growth of rMVEV and NS1-2A cleavage site mutants in mammalian and insect cells. Vero and C6/36 cells were infected at a multiplicity of ~0.1. Virus titers in growth samples were measured by plaque assay on Vero cells. The interrupted line denotes the detection limit of virus yield. Error bars indicate the standard error of the mean (SEM) of two independent experiments. b Virus-specific RNA in infected Vero cells. Vero cells were infected with rMVEV or NS1-2A cleavage site mutants (MOI ~ 1) and total intracellular RNA extracted at the indicated time points. Virus-specific RNA was measured by real-time qRT-PCR. Error bars indicate the SEM for two determinations for each sample

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