Fig. 3

The sensitivity comparison of CPA-LFD, conventional RT-PCR and qRT-PCR. a CPA-LFD; b conventional RT-PCR; c qRT-PCR. The amplification curve 1–7, the concentrations of RGNNV RNA1 standard in the reaction solution was 106, 105, 104, 103, 102, 101, 100 copies/μL, respectively. N: negative control