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Table 1 Effects of double-substitutions on cell-to-cell movement, long-distance spread and replication

From: The multifunctional protein CI of potyviruses plays interlinked and distinct roles in viral genome replication and intercellular movement

Mutants Residues substituted Cell-to-cell spread Long-distance movement Replication Relative levels of viral RNA
CI-m1 D4, E6 - - + 1.08 ± 0.14
CI-m2 D7, D8 - - + 1.03 ± 0.18
CI-m3 E11, R12 - - reduced** 0.72 ± 0.08
CI-m4 D17, E19 - - + 1.15 ± 0.17
CI-m5 D23, D25 slow + + 0.93 ± 0.13
CI-m6 E33, K34 slow + + 1.05 ± 0.09
CI-m7 E56, K58 + + + 1.02 ± 0.14
CI-m8 E77, D79 + + + 0.93 ± 0.07
CI-m9 K80, D81 - - + 1.04 ± 0.14
CI-m10 R102, K103 - - reduced* 0.90 ± 0.03
CI-m11 E167, E168 + + + 0.86 ± 0.10
CI-m12 D175, E176 - - - 0.52 ± 0.03
CI-m13 E193, D195 slow + reduced* 0.79 ± 0.09
CI-m14 R210, E211 - - - 0.49 ± 0.09
CI-m15 E225, D226 slow + reduced* 0.84 ± 0.08
CI-m16 K248, D250 - - - 0.62 ± 0.12
CI-m17 E261, D263 slow - reduced* 0.81 ± 0.06
CI-m18 E271, D273 + + reduced** 0.82 ± 0.04
CI-m19 K278, D280 - - + 1.01 ± 0.18
CI-m20 K299, K300 slow + reduced** 0.83 ± 0.05
CI-m21 D316, D318 - - + 0.95 ± 0.08
CI-m22 D332, D334 slow + + 0.89 ± 0.11
CI-m23 E349, R350 - - - 0.52 ± 0.09
CI-m24 E372, K373 slow + + 0.87 ± 0.13
CI-m25 R433, D435 slow + + 0.90 ± 0.14
CI-m26 K447, R448 slow - reduced* 0.73 ± 0.08
CI-m27 K450, R452 - - reduced** 0.72 ± 0.06
CI-m28 D453, E455 - - reduced** 0.68 ± 0.04
CI-m29 E485, D486 + + + 1.06 ± 0.04
CI-m30 R487, R488 + + + 0.98 ± 0.15
CI-m31 D489, R491 slow - reduced* 0.81 ± 0.10
CI-m32 R497, D498 - - - 0.50 ± 0.10
CI-m33 E501, K502 - - + 1.02 ± 0.12
CI-m34 K513, K515 - - + 1.02 ± 0.16
CI-m35 E555, E556 slow - reduced* 0.80 ± 0.09
CI-m36 R557, R558 - - reduced** 0.64 ± 0.05
CI-m37 K559, E561 - - reduced** 0.77 ± 0.03
CI-m38 K591, D592 slow - reduced* 0.71 ± 0.12
CI-m39 K623, D625 slow + + 0.92 ± 0.08
CI-m40 K92, S93 - - increased* 1.34 ± 0.17
GDD   - - - 0.58 ± 0.09
WT   + + + 1
  1. Note: +, similar to the wild type virus; −, replication or cell-to-cell movement or long distance spread was abolished; slow, reduced spread intercellularly in the inoculated leaves; reduced, replication significantly reduced; increased, replication significantly enhanced; *, P < 0.05; **, P < 0.01