|
Mutants
|
Residues substituted
|
Cell-to-cell spread
|
Long-distance movement
|
Replication
|
Relative levels of viral RNA
|
|---|
|
CI-m1
|
D4, E6
|
-
|
-
|
+
|
1.08 ± 0.14
|
|
CI-m2
|
D7, D8
|
-
|
-
|
+
|
1.03 ± 0.18
|
|
CI-m3
|
E11, R12
|
-
|
-
|
reduced**
|
0.72 ± 0.08
|
|
CI-m4
|
D17, E19
|
-
|
-
|
+
|
1.15 ± 0.17
|
|
CI-m5
|
D23, D25
|
slow
|
+
|
+
|
0.93 ± 0.13
|
|
CI-m6
|
E33, K34
|
slow
|
+
|
+
|
1.05 ± 0.09
|
|
CI-m7
|
E56, K58
|
+
|
+
|
+
|
1.02 ± 0.14
|
|
CI-m8
|
E77, D79
|
+
|
+
|
+
|
0.93 ± 0.07
|
|
CI-m9
|
K80, D81
|
-
|
-
|
+
|
1.04 ± 0.14
|
|
CI-m10
|
R102, K103
|
-
|
-
|
reduced*
|
0.90 ± 0.03
|
|
CI-m11
|
E167, E168
|
+
|
+
|
+
|
0.86 ± 0.10
|
|
CI-m12
|
D175, E176
|
-
|
-
|
-
|
0.52 ± 0.03
|
|
CI-m13
|
E193, D195
|
slow
|
+
|
reduced*
|
0.79 ± 0.09
|
|
CI-m14
|
R210, E211
|
-
|
-
|
-
|
0.49 ± 0.09
|
|
CI-m15
|
E225, D226
|
slow
|
+
|
reduced*
|
0.84 ± 0.08
|
|
CI-m16
|
K248, D250
|
-
|
-
|
-
|
0.62 ± 0.12
|
|
CI-m17
|
E261, D263
|
slow
|
-
|
reduced*
|
0.81 ± 0.06
|
|
CI-m18
|
E271, D273
|
+
|
+
|
reduced**
|
0.82 ± 0.04
|
|
CI-m19
|
K278, D280
|
-
|
-
|
+
|
1.01 ± 0.18
|
|
CI-m20
|
K299, K300
|
slow
|
+
|
reduced**
|
0.83 ± 0.05
|
|
CI-m21
|
D316, D318
|
-
|
-
|
+
|
0.95 ± 0.08
|
|
CI-m22
|
D332, D334
|
slow
|
+
|
+
|
0.89 ± 0.11
|
|
CI-m23
|
E349, R350
|
-
|
-
|
-
|
0.52 ± 0.09
|
|
CI-m24
|
E372, K373
|
slow
|
+
|
+
|
0.87 ± 0.13
|
|
CI-m25
|
R433, D435
|
slow
|
+
|
+
|
0.90 ± 0.14
|
|
CI-m26
|
K447, R448
|
slow
|
-
|
reduced*
|
0.73 ± 0.08
|
|
CI-m27
|
K450, R452
|
-
|
-
|
reduced**
|
0.72 ± 0.06
|
|
CI-m28
|
D453, E455
|
-
|
-
|
reduced**
|
0.68 ± 0.04
|
|
CI-m29
|
E485, D486
|
+
|
+
|
+
|
1.06 ± 0.04
|
|
CI-m30
|
R487, R488
|
+
|
+
|
+
|
0.98 ± 0.15
|
|
CI-m31
|
D489, R491
|
slow
|
-
|
reduced*
|
0.81 ± 0.10
|
|
CI-m32
|
R497, D498
|
-
|
-
|
-
|
0.50 ± 0.10
|
|
CI-m33
|
E501, K502
|
-
|
-
|
+
|
1.02 ± 0.12
|
|
CI-m34
|
K513, K515
|
-
|
-
|
+
|
1.02 ± 0.16
|
|
CI-m35
|
E555, E556
|
slow
|
-
|
reduced*
|
0.80 ± 0.09
|
|
CI-m36
|
R557, R558
|
-
|
-
|
reduced**
|
0.64 ± 0.05
|
|
CI-m37
|
K559, E561
|
-
|
-
|
reduced**
|
0.77 ± 0.03
|
|
CI-m38
|
K591, D592
|
slow
|
-
|
reduced*
|
0.71 ± 0.12
|
|
CI-m39
|
K623, D625
|
slow
|
+
|
+
|
0.92 ± 0.08
|
|
CI-m40
|
K92, S93
|
-
|
-
|
increased*
|
1.34 ± 0.17
|
|
GDD
| |
-
|
-
|
-
|
0.58 ± 0.09
|
|
WT
| |
+
|
+
|
+
|
1
|
- Note: +, similar to the wild type virus; −, replication or cell-to-cell movement or long distance spread was abolished; slow, reduced spread intercellularly in the inoculated leaves; reduced, replication significantly reduced; increased, replication significantly enhanced; *, P < 0.05; **, P < 0.01
