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Table 1 Effects of double-substitutions on cell-to-cell movement, long-distance spread and replication

From: The multifunctional protein CI of potyviruses plays interlinked and distinct roles in viral genome replication and intercellular movement

Mutants

Residues substituted

Cell-to-cell spread

Long-distance movement

Replication

Relative levels of viral RNA

CI-m1

D4, E6

-

-

+

1.08 ± 0.14

CI-m2

D7, D8

-

-

+

1.03 ± 0.18

CI-m3

E11, R12

-

-

reduced**

0.72 ± 0.08

CI-m4

D17, E19

-

-

+

1.15 ± 0.17

CI-m5

D23, D25

slow

+

+

0.93 ± 0.13

CI-m6

E33, K34

slow

+

+

1.05 ± 0.09

CI-m7

E56, K58

+

+

+

1.02 ± 0.14

CI-m8

E77, D79

+

+

+

0.93 ± 0.07

CI-m9

K80, D81

-

-

+

1.04 ± 0.14

CI-m10

R102, K103

-

-

reduced*

0.90 ± 0.03

CI-m11

E167, E168

+

+

+

0.86 ± 0.10

CI-m12

D175, E176

-

-

-

0.52 ± 0.03

CI-m13

E193, D195

slow

+

reduced*

0.79 ± 0.09

CI-m14

R210, E211

-

-

-

0.49 ± 0.09

CI-m15

E225, D226

slow

+

reduced*

0.84 ± 0.08

CI-m16

K248, D250

-

-

-

0.62 ± 0.12

CI-m17

E261, D263

slow

-

reduced*

0.81 ± 0.06

CI-m18

E271, D273

+

+

reduced**

0.82 ± 0.04

CI-m19

K278, D280

-

-

+

1.01 ± 0.18

CI-m20

K299, K300

slow

+

reduced**

0.83 ± 0.05

CI-m21

D316, D318

-

-

+

0.95 ± 0.08

CI-m22

D332, D334

slow

+

+

0.89 ± 0.11

CI-m23

E349, R350

-

-

-

0.52 ± 0.09

CI-m24

E372, K373

slow

+

+

0.87 ± 0.13

CI-m25

R433, D435

slow

+

+

0.90 ± 0.14

CI-m26

K447, R448

slow

-

reduced*

0.73 ± 0.08

CI-m27

K450, R452

-

-

reduced**

0.72 ± 0.06

CI-m28

D453, E455

-

-

reduced**

0.68 ± 0.04

CI-m29

E485, D486

+

+

+

1.06 ± 0.04

CI-m30

R487, R488

+

+

+

0.98 ± 0.15

CI-m31

D489, R491

slow

-

reduced*

0.81 ± 0.10

CI-m32

R497, D498

-

-

-

0.50 ± 0.10

CI-m33

E501, K502

-

-

+

1.02 ± 0.12

CI-m34

K513, K515

-

-

+

1.02 ± 0.16

CI-m35

E555, E556

slow

-

reduced*

0.80 ± 0.09

CI-m36

R557, R558

-

-

reduced**

0.64 ± 0.05

CI-m37

K559, E561

-

-

reduced**

0.77 ± 0.03

CI-m38

K591, D592

slow

-

reduced*

0.71 ± 0.12

CI-m39

K623, D625

slow

+

+

0.92 ± 0.08

CI-m40

K92, S93

-

-

increased*

1.34 ± 0.17

GDD

 

-

-

-

0.58 ± 0.09

WT

 

+

+

+

1

  1. Note: +, similar to the wild type virus; −, replication or cell-to-cell movement or long distance spread was abolished; slow, reduced spread intercellularly in the inoculated leaves; reduced, replication significantly reduced; increased, replication significantly enhanced; *, P < 0.05; **, P < 0.01
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