Fig. 4

Analysis of monoclonal antibody sensitivity through ACP-ELISA. BYDV GAV-infected and healthy (CK-) wheat leaf crude extracts were two-fold diluted [1:20 to 1:655,360 (w/v, g · mL⁻¹)] in a 0.05 mM sodium bicarbonate buffer and 100 μl diluted wheat extract was loaded into each well on the ELISA plate. Monoclonal antibody 18A1, 18A9 and 12A11 were diluted 1:6,000, 1:5,000 and 1:5,000 prior to the assay. Each OD₄₀₅ value represents the mean of three independent assays at 40 min post addition of the substrate at room temperature