Fig. 2

Identification of potential Gag proximal and interacting partners. Jurkat cells were transfected with BirA* or BirA*-Gag in medium supplemented with 50 μM Biotin (Sigma). Cell lysates were harvested 24 h post-transfection. Dynabeads MyOne Streptavidin C1 (Life Technologies) were used to precipitate biotinylated proteins from 1 mg of lysate as described by the manufacturer. The eluted proteins were separated on 4-15 % SDS-PAGE (Bio-Rad), the bands excised and analyzed by LC-tandem MS at SAMS Centre for proteomics, University of Calgary. a Venn diagram depicting the number of overlapping hits between Gag-interacting proteins. b Genes families and subfamilies were grouped and classified, using PANTHER, according to their function in three different ways: biological process, molecular function and protein class. c GeneMANIA (used in automatically selected weighting method) was used to determine known interactions between input genes and how they interact. The relationships between genes in the network included physical and genetic interactions, pathways and protein domain similarity