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Table 1 Summary of transposon-mediated gene interruptions within the coding regions of the MeHV-1 infectious clone pMeHV1-C18. The genes affected and encoded gene products are shown

From: Identification of non-essential loci within the Meleagrid herpesvirus 1 genome

Gene/element

Gene product/function

pMeHV1-C18

GaHV-21

HHV-12

HHV-33

SuHV-14

BoHV-15

vLip

Viral lipase

NE

NE

NA

NA

NA

NA

LORF2

Unknown

NE

E/NE

NA

NA

NA

NA

UL6

Portal protein

E

ND

E

E

ND

E

UL8

Helicase/ primase associated protein

E

ND

E

E

ND

E

UL9

Origin binding protein

E

ND

E

E

E

E

UL10

Glycoprotein M

NE

E

NE

E

E

NE

UL13

Protein kinase

A

NE

NE

NE

NE

NE

UL17

Tegument/DNA packaging protein

E

E

E

E

E

E

UL19

VP5 capsid protein

E

E

E

E

NE

E

UL21

Tegument protein

A

ND

NE

E

NE

NE

UL26

Scaffold protease

E

ND

E

E

ND

E

UL26.5

Scaffold protein

E

ND

E

E

ND

E

UL27

Glycoprotein B

E*

E

E

E

ND

E

UL29

Single stranded DNA binding protein

E

ND

E

E

E

E

UL36

Large tegument protein

E

E

E

E

E

E

UL37

Tegument protein

E

E

E

E

ND

E

UL39

Ribonucleotide reductase large subunit

NE

NE

NE

NE

NE

NE

UL40

Ribonucleotide reductase small subunit

NE*

ND

NE

NE

ND

NE

UL42

DNA polymerase processivity subfactor

E

ND

E

E

ND

E

UL47

VP13/14 capsid protein

NE

NE

NE

NE

NE

NE

UL48

VP16 α-transinducing factor

NE

NE

E

NE

NE

NE

UL52

Helicase/ primase associated protein

E

ND

E

E

ND

E

UL53

Glycoprotein K

A

E

NE

E

E

E

LORF4A

Unknown

NE

ND

NA

NA

NA

NA

LORF5

Unknown

NE#

NE

NA

NA

NA

NA

icp4

Major immediate early regulatory gene

E

ND

E

E

ND

ND

US3

Protein kinase

NE

NE

NE

E

NE

NE

US6

Glycoprotein D

NE*

NE

E

ND

NE

E

  1. The gene requirements for pMeHV1-C18 determined in this study are designated as either essential (E), non-essential (NE) or severely attenuated (A). Comparative data, using the same nomenclature, is shown for selected alphaherpesviruses; Gallid herpesvirus 2 (GaHV-2), Human herpesvirus 1 (HHV-1), Human herpesvirus 3 (HHV-3), Suid herpesvirus 1 (SuHV-1) and Bovine herpesvirus 1 (BoHV-1). Not applicable (NA) denotes genes unique to the genus Mardivirus. Rows in bold highlight those genes for which requirement varies between the viruses listed. *Requirement for growth in cell culture previously reported in wildtype MeHV-1 virus [57–59]
  2. #Transposon was unstable in viral genome
  3. References for gene interruption studies: 1[18, 23, 25, 42, 49, 57, 60–67]; 2[14, 35, 38, 58, 68–76]; 3[26, 41, 45, 77, 78]; 4[17, 27, 43, 48, 79–82]; 5[19, 83]