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Table 1 Summary of transposon-mediated gene interruptions within the coding regions of the MeHV-1 infectious clone pMeHV1-C18. The genes affected and encoded gene products are shown

From: Identification of non-essential loci within the Meleagrid herpesvirus 1 genome

Gene/element Gene product/function pMeHV1-C18 GaHV-21 HHV-12 HHV-33 SuHV-14 BoHV-15
vLip Viral lipase NE NE NA NA NA NA
LORF2 Unknown NE E/NE NA NA NA NA
UL6 Portal protein E ND E E ND E
UL8 Helicase/ primase associated protein E ND E E ND E
UL9 Origin binding protein E ND E E E E
UL10 Glycoprotein M NE E NE E E NE
UL13 Protein kinase A NE NE NE NE NE
UL17 Tegument/DNA packaging protein E E E E E E
UL19 VP5 capsid protein E E E E NE E
UL21 Tegument protein A ND NE E NE NE
UL26 Scaffold protease E ND E E ND E
UL26.5 Scaffold protein E ND E E ND E
UL27 Glycoprotein B E* E E E ND E
UL29 Single stranded DNA binding protein E ND E E E E
UL36 Large tegument protein E E E E E E
UL37 Tegument protein E E E E ND E
UL39 Ribonucleotide reductase large subunit NE NE NE NE NE NE
UL40 Ribonucleotide reductase small subunit NE* ND NE NE ND NE
UL42 DNA polymerase processivity subfactor E ND E E ND E
UL47 VP13/14 capsid protein NE NE NE NE NE NE
UL48 VP16 α-transinducing factor NE NE E NE NE NE
UL52 Helicase/ primase associated protein E ND E E ND E
UL53 Glycoprotein K A E NE E E E
LORF4A Unknown NE ND NA NA NA NA
LORF5 Unknown NE# NE NA NA NA NA
icp4 Major immediate early regulatory gene E ND E E ND ND
US3 Protein kinase NE NE NE E NE NE
US6 Glycoprotein D NE* NE E ND NE E
  1. The gene requirements for pMeHV1-C18 determined in this study are designated as either essential (E), non-essential (NE) or severely attenuated (A). Comparative data, using the same nomenclature, is shown for selected alphaherpesviruses; Gallid herpesvirus 2 (GaHV-2), Human herpesvirus 1 (HHV-1), Human herpesvirus 3 (HHV-3), Suid herpesvirus 1 (SuHV-1) and Bovine herpesvirus 1 (BoHV-1). Not applicable (NA) denotes genes unique to the genus Mardivirus. Rows in bold highlight those genes for which requirement varies between the viruses listed. *Requirement for growth in cell culture previously reported in wildtype MeHV-1 virus [5759]
  2. #Transposon was unstable in viral genome
  3. References for gene interruption studies: 1[18, 23, 25, 42, 49, 57, 6067]; 2[14, 35, 38, 58, 6876]; 3[26, 41, 45, 77, 78]; 4[17, 27, 43, 48, 7982]; 5[19, 83]