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Fig. 2 | Virology Journal

Fig. 2

From: Identification of non-essential loci within the Meleagrid herpesvirus 1 genome

Fig. 2

Restriction fragment length polymorphism of icp4 insertion mutants a Schematic representation of the four potential genotypes resulting from recombination between the IRS/TRS elements of pMeHV1-C18-Tn5Δ-1 during virus replication. Genotype 1: Transposon in TRS; Genotype 2: Inversion of transposon to the IRS; Genotype 3: Duplication of transposon; Genotype 4: Loss of transposon. (b) In silico FseI/SbfI restriction endonuclease digestion patterns for pMeHV1-C18-Tn5Δ1 Genotypes 1, 2, 3, and 4. (c) iBAC DNA co-digested with FseI and SbfI. lane 1: pMeHV1-C18-Tn5Δ1 (genotype 1); lane 2: loss of transposon (genotype 4); lane 3: transposon in IRS element (genotype 2); lane 4: transposon in TRS element (genotype 1); lane 5: duplication of transposon (genotype 3)

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