Table 1 RT-qPCR primers
From: Differential expression of microRNAs in porcine parvovirus infected porcine cell line
Gene | RT primer | |
|---|---|---|
U6 | 5’CGCTTCACGAATTTGCGTGTCAT3’ | |
miR-RT Primer | 5’GTCGGTGTCGTGGAGTCGTTTGCAATTGCACTGGATTTTTTTTTTTTTTTTTTV3’ | |
V = A, G, C | ||
Gene | Forward primer (5’–3’) | Reversed primer (5’–3’) |
ssc-miR-10b | TACCCTGTAGAACCGAATTTGT | GTCGGTGTCGTGGAGTCG |
ssc-miR-30a-5p | TGTAAACATCCTCGACTGGAAG | GTCGGTGTCGTGGAGTCG |
ssc-miR-16 | TAGCAGCACGTAAATATTGGC | GTCGGTGTCGTGGAGTCG |
ssc-miR-17-5p | CAAAGTGCTTACAGTGCAGGTAG | GTCGGTGTCGTGGAGTCG |
ssc-miR-192 | CTGACCTATGAATTGACA | GTCGGTGTCGTGGAGTCG |
ssc-miR-21 | TAGCTTATCAGACTGATGTTGA | GTCGGTGTCGTGGAGTCG |
ssc-miR-19b | TGTGCAAATCCATGCAAAAC | GTCGGTGTCGTGGAGTCG |
ssc-miR-18a | TAAGGTGCATCTAGTGCAGATA | GTCGGTGTCGTGGAGTCG |
ssc-miR-152 | TCAGTGCATGACAGAACTTGG | GTCGGTGTCGTGGAGTCG |
ssc-miR-novel-chr13_10861 | TTCAAGTAACCCAGGATAGGCT | GTCGGTGTCGTGGAGTCG |
U6 | TCGCTTTGGCAGCACCTAT | AATATGGAACGCTTCGCAAA |