Fig. 5

Accumulation of a Myc-tagged version of the A. thaliana AGO1 protein transiently expressed in N. benthamiana leaves in the presence or absence of P0 proteins. Myc-tagged versions of cotton leafroll dwarf virus P0s (P0^CL-ARG, P0^CL-PV1, P0^CL-Acr9, P0^CL-Hol1, P0^CL-Ima2, P0^CL-Ipa4, P0^CL-Pal3 or P0^CL-Pm1) and potato leafroll virus P0 (P0^PL-AU) were used in the assay. Non-infiltrated plants (NI) were used as negative controls and AtAGO1 infiltrated with the empty vector or with a Myc-tagged P19 were used as positive controls. Membranes were probed with anti-Myc antibodies. Ponceau staining was used as a loading control. All co-infiltrations were performed in the presence of P19 (without tag) to stabilize mRNA Agrobacterium-mediated experiments