Fig. 3

Suppression of local silencing by cotton leafroll dwarf virus P0s in N. benthamiana 16c plants. Transgenic N. benthamiana plants expressing GFP (line 16c) were co-infiltrated with Agrobacterium carrying plasmids to express GFP (silencing trigger) and candidate suppressor proteins (P0^CL-PV1, P0^CL-Acr9, P0^CL-Hol1, P0^CL-Ima2, P0^CL-Ipa4, P0^CL-Pal3 and P0^CL-Pm1). GFP co-expressed with empty vector was used as negative control. P0^CL-ARG, P0^PL-AU and P19 were used as positive controls in the assay. Pictures were taken at 3, 6 and 9 days post-infiltration (dpi)