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Table 1 Primers for PCR and sequencing

From: Construction of a recombinant duck enteritis virus (DEV) expressing hemagglutinin of H5N1 avian influenza virus based on an infectious clone of DEV vaccine strain and evaluation of its efficacy in ducks and chickens

Primer

Sequence

Kan ins’ H5(HA) F

5′-TTAgagctcCTCGCTGCAGGCGGCCGCTCTAGAACTCGTCGATCGCAGCGGGATGACGACGATAAGTAGGGATAAC-3′ a

Kan ins’ H5(HA) R

5′-CGCgagctcGGGTAATGCCAGTGTTACAACCA-3′ a

DEV ins H5 casse UL55 F

5′-CGACGGACTGCCAGTGAACGCTGAACAAGCTAGGACAATTCTAGTGGATCCCCCAACTCC-3′

DEV ins H5 casse UL55 R

5′-AAGTAAAGACCCAAGCTACTAACAGGGTATTTGGGTAATATTGTCGACTCTAGAGGATCCG-3′

DEV gC flanking F

5′-TTCGCCGTATTTACCAAATG-3′

DEV gC flanking R

5′-TGATTCCTTTTGTTCGGATA-3′

DEV H5(UL55) casse seq F1

5′-CTAGTGGATCCCCCAACTCC-3′

DEV H5(UL55) casse seq F2

5′-GTACATTGGGTCAATGGGAG-3′

DEV H5(UL55) casse seq F3

5′-AAGTACACTGCGTCAATAGG-3′

DEV H5(UL55) casse seq F4

5′-ACTTTCCAATGGGTTTTGCC-3′

DEV H5(UL55) casse seq F5

5′-GCTGATTAATGGGAAAGTAC-3′

DEV H5(UL55) casse seq F6

5′-CGATCATATTTGCATTGGTT-3′

DEV H5(UL55) casse seq F7

5′-TTGAAACACCTATTGAGCAG-3′

DEV H5(UL55) casse seq F8

5′-GGACATCAACACTAAACCAG-3′

DEV H5(UL55) casse seq F9

5′-GAATGTCCCAAATATGTGAA-3′

DEV H5(UL55) casse seq F10

5′-CTTAGAGAGGAGAATAGAGAAT-3′

DEV H5(UL55) casse seq R1

5′-TTGTCGACTCTAGAGGATCC-3′

DEV H5(UL55) casse seq R2

5′-CATCCATAAAGATAGACCAG-3′

DEV H5(UL55) casse seq R3

5′-ATGGAAGTCTAGAGTTCTCTC-3′

DEV H5(UL55) casse seq R4

5′-TAAAACCTGCTATAGCTCCA-3′

DEV H5(UL55) casse seq R5

5′-CGGTTTTAAAATTGTCCAGA-3′

DEV H5(UL55) casse seq R6

5′-TGGACATGCTGCACTCACCC-3′

DEV H5(UL55) casse seq R7

5′-TTTCCAGTATGTCTTGGGCA-3′

DEV H5(UL55) casse seq R8

5′-ATATGGAATTTCCAGGGGAA-3′

DEV H5(UL55) casse seq R9

5′-CCCAATGGAAAGTCCCTATT-3′

DEV H5(UL55) casse seq R10

5′-TCAGTGTACTTGGCTCCAAT-3′

  1. a restriction enzyme sites added to primers are in bold lower case letters, and sequences in italics indicate additional bases that are not present in the original DEV genome