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Fig. 3 | Virology Journal

Fig. 3

From: Construction of a recombinant duck enteritis virus (DEV) expressing hemagglutinin of H5N1 avian influenza virus based on an infectious clone of DEV vaccine strain and evaluation of its efficacy in ducks and chickens

Fig. 3

Construction of recombinant pDEVC-KCE-H5(UL55). a A kanamycin resistance gene (sm) with a 40 bp homologous sequence (c) was inserted into the Sac I restriction site (*) in the HA expression cassette, which was divided into two parts (HA Cas’ and HA Cas”). b The HA expression cassette with the selective marker (sm) was inserted into the noncoding area between LORF11 and UL55 through the first recombination to generate a recombinant BAC clone (pDEVC-KCE-H5(UL55)KAN) with both chloramphenicol and kanamycin resistance. c The second recombination was performed to delete the kanamycin resistance gene and generate the final recombinant pDEVC-KCE-H5(UL55)-vectored HA clone. Rectangles with the same type of shading indicate identical sequences. Scales in bp or kbp are provided

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