Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: Construction of a recombinant duck enteritis virus (DEV) expressing hemagglutinin of H5N1 avian influenza virus based on an infectious clone of DEV vaccine strain and evaluation of its efficacy in ducks and chickens

Fig. 2

RFLP of DEVC-KCE BAC clone Sa, of pDEVC-KCE-H5(UL55)KAN, and of pDEVC-KCE-H5(UL55). a DNA from DEVC-KCE BAC clone Sa (lanes 1 and 4) and recombinant BACs of pDEVC-KCE-H5(UL55)KAN (lanes 2 and 5) and pDEVC-KCE-H5(UL55) (lanes 3 and 6) were prepared by mini-prep and digested with BamH I (lanes 1–3) or EcoR I (lanes 4–6). The digests were separated by 0.8 % agarose gel electrophoresis for 16 h under 40v. Arrowheads highlight differences between the lanes. b Predicted RFLP patterns of pDEVC-KCE-H5(UL55)KAN and pDEVC-KCE-H5(UL55) compared with BAC clone Sa. Size markers range from 2 to 20 kb. Lanes 1–3 show predicted BamH I digestion patterns of Sa, pDEVC-KCE-H5(UL55)KAN, and pDEVC-KCE-H5(UL55), respectively. Lanes 4–6 show predicted EcoR I digestion patterns of Sa, pDEVC-KCE-H5(UL55)KAN, and pDEVC-KCE-H5(UL55), respectively. Predictions of these digestions were performed using the DEV (VAC) whole genome sequences as a reference (GenBank ID:EU082088.2). M: DL 15,000 DNA Marker (Takara)

Back to article page