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Fig. 1 | Virology Journal

Fig. 1

From: Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens

Fig. 1

Production of chicken IL-2 was assessed by western blot in vitro (DF-1 cell line) and in vivo (infected eggs) at 48 hpi. A strong band of approximately 15 kDa is present in blots from the supernatant (panel a), cell lysate (panel b), and allantoic fluid (panel b) of rZJ1-IL2-infected DF-1 cells and eggs. No band of the same molecular weight was present in blots from the rZJ1-GFP-infected groups. Blots from the supernatant (panel a), cell lysates (panel b), and allantoic (panel b) fluid of both rZJ1-IL2 and rZJ1-GFP groups showed bands for NDV NP at approximately 50 kDa. No bands were observed in mock-inoculated controls, either at 15 or 50 kDa

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