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Table 3 Repeated experiments using indirect ELISA kits of different batch (OD value)

From: Analysis of TTSuV1b antibody in porcine serum and its correlation with four antibodies against common viral infectious diseases

Antigens from different batch

Positive serum Inter-batch variation

Negative serum Inter-batch variation

1

2

3

4

1

2

3

4

1

1.172

0.983

1.025

0.989

0.158

0.131

0.137

0.130

2

1.090

0.969

1.046

1.071

0.171

0.152

0.134

0.132

3

1.105

1.040

1.159

1.081

0.166

0.131

0.115

0.116

4

1.038

1.022

1.042

1.091

0.165

0.157

0.119

0.122

5

1.072

1.005

1.095

1.004

0.147

0.142

0.125

0.116

MEAN

1.095

1.004

1.073

1.035

0.161

0.143

0.126

0.123

STDEV

0.049

0.028

0.054

0.044

0.009

0.011

0.009

0.007

CV (%)

4.50Ā %

2.86Ā %

5.08Ā %

4.27Ā %

5.77Ā %

8.31Ā %

7.49Ā %

6.15Ā %

Mean value of CV

4.18Ā %

6.91Ā %