Fig. 2
From: The Chinese herb-derived Sparstolonin B suppresses HIV-1 transcription
SsnB inhibited HIV LTR promoter activity. a 293T cells were transfected with HIV LTR plasmid together with pGL4.74 [hRluc/TK]. Cells were either left unstimulated or stimulated with PMA (50 ng/ml) for 12 h followed by mock or SsnB (1 μg/ml, ~3.7 μM) treatment for another 12 h. Luciferase assay was performed. Normalized HIV LTR promoter activity was presented. b CEM-SS cells were treated with SsnB at various concentrations for 12 h and cell viability was determined by CellTiter Glo kit (Promega) 24 h after the initial exposure