Fig. 6

Immunohistochemical detection of PCV2 antigen in conventional pigs. a No detection of PCV2 antigen in lymph nodes of mock pigs. b Very small amounts of PCV2 antigen in lymph nodes of a pig vaccinated with 2×104.0 TCID50 dose attenuated PCV1-2b. c Low amounts of PCV2 antigen in nuclei of histiocytes and occasional lymphocytes of a pig vaccinated with inactivated PCV1-2b. d Small amounts of PCV2 antigen in nuclei of histiocytes and occasional lymphocytes of a pig vaccinated with 2×103.5 TCID50 dose attenuated PCV1-2b. e Moderate amounts of PCV2 antigen in nuclei of histiocytes and occasional lymphocytes of a pig vaccinated with commercial inactivated PCV2b vaccine X. f High amounts of PCV2 antigen in nuclei of histiocytes and occasional lymphocytes of a nonvaccinated challenged pig. Bar = 20 μm (400×). Hematoxylin-counterstained sections