Fig. 2

The stem-loop formed by nucleotide 142 to 170 in 5’UTR of HIV-1 genomic RNA is important for interaction with eEF1A. a The predicted secondary structure of HIV-1 5’UTR is formed by TAR, polyA, tRNA anticodon-like element (TLE), PBS and stem-loop 1, 2, 3 (SL1, 2, 3). The arrows indicate the sites of truncated RNA. b Biotin labelled wild-type, truncated and mutated 5’UTR RNAs were immobilized on biosensors. The maximum responses (nm) during a 600 s incubation of each biosenor with 90 nM of eEF1A protein using OctetRed system are shown. c The local secondary structures of wild-type, loop and bulge mutated RNAs and their minimum free energy (ΔG) were predicted using Mfold [21]. All data sets are presented as mean ± SD from at least 3 independent experiments and * indicates p < 0.05