Fig. 2
From: DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells
The localization of HPV replication foci in U2OS cells in relation to the DAXX protein. a and b Immunofluorescence analysis of U2OS cells transfected with HPV11 genome. U2OS cells were transfected with wt HPV11 minicircle genomes and grown on coverslips. Six days after transfection cells were fixed and immunostained with antibodies for HPV11 E2 (green) and DAXX (red) proteins. Cell nuclei were detected by DAPI. Analysis was carried out at least three times starting from cell transfections. a and b represent cells with different number and size of HPV11 E2 foci. c FISH analysis of U2OS cells transfected with HPV18 genome. U2OS cells were transfected with wt HPV18 minicircle genomes and grown on microscope slides. Six days after transfection cells were fixed and combined immunofluorescence and FISH analysis was performed. DAXX signal is visible in red and HPV18 DNA in green. Cell nuclei were detected by DAPI. Analysis was carried out at least three times starting from cell transfections. On the right panels, the intensity profiles are shown