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Table 2 Testing clinical specimens to evaluate the performance of Monoplex and Multiplex PCR assays

From: Multiplex PCR for rapid diagnosis and differentiation of pox and pox-like diseases in dromedary Camels

Country/area/year

No. of skin specimens

Suspected virus

Monoplex PCR positive specimens (%)

Multiplex PCR positive specimens (%)

Positivity by standard methods

Sudan/Showak/2000

6

CMLV

6 (100)

6 (100)

ND*

Sudan/Showak/2013

8

CMLV

6 (75)

6 (75)

4 (50)

SaudiArabia/Hail/2014

3

CMLV

3 (100)

3 (100)

3 (100)

Sudan/Showak/1993

6

CPPV

4 (66.7)

4 (66.7)

3 (50)

Sudan/Showak/2000

8

CPPV

7 (87.5)

7 (87.5)

ND

Sudan/Showak/2005

6

CPPV

6 (100)

6 (100)

ND

Sudan/Showak/2012

6

CPPV

4 (66.7)

4 (66.7)

ND

Sudan/Showak/2013

5

CPPV

3 (60)

3 (60)

ND

Sudan/Showak/2013

8

CPPV

6 (75)

6 (75)

ND

Saudi Arabia/Hail/2014

3

CPPV

0

0

ND

Sudan/Showak/1994

5

CdPV

5 (100)

5 (100)

ND

Sudan/Khartoum/2009

6

CdPV

5 (83.3)

5 (83.3)

ND

Sudan/Showak/2012

4

CdPV

3 (75)

3 (75)

ND

Sudan/Showak/2013

3

CdPV

3 (100)

3 (100)

ND

Saudi Arabia/Al-Ahsa/2013

3

CdPV

0

0

ND

Saudi Arabia/Al-Ahsa/2014

6

CdPV

4 (66.7)

4 (66.7)

4

Total

86

 

65 (75.6)

65 (66.7)

 
  1. ND: not done