Fig. 6

Validation of rice gene expression levels by real time RT-PCR and Northern blotting. a Comparison of protein and mRNA expression levels of mock leaves and RSV-infected leaves using RT-qPCR. Blue represents mock leaves; red represents RSV-infected leaves. The averaged readings from the three biological replicates normalized against endogenous gene OsEF1α; error bar denoted SD. Statistics were analyzed using the Student’s t-test. An asterisk indicated a significant difference from the corresponding control (P < 0.01). b Northern blot of two differentially expressed genes selected for verifying RT-qPCR results. Mock, mock-inoculated leaves; RSV-infected, RSV-infected leaves. Equal loading of total RNA was assessed by staining rRNA with ethidium bromide. Marker contained 2000 bp, 1500 bp, 1000 bp and 750 bp